01
Select optical input
INPUTS
Optical inputs
Lens aperture + lateral magnification
Lens aperturef/4.00Enter the marked f-number (for example, f/4).
Lateral magnification (optional)Not specifiedIf omitted, the high-magnification limiting case is used.
Magnification not specified: the calculation uses the limiting case m → ∞ and assumes a pupil magnification of 1. The reported DOF is a model-based lower bound.
02
Calculation wavelength
Shorter wavelength → smaller diffraction-limited DOF
λ550nm
Green · white-light reference
For broadband white-light imaging, 550 nm is used as a representative wavelength. Select 450 or 435 nm only when the illumination or spectral filter is centered in the blue/violet band.
03
Focus-stack parameters
DOF-overlap preset17.806 µm
Target axial range0.1 mmUnit: mm